What question did this study set out to answer?

To identify the enzymatic mechanism by which the Campylobacter jejuni cytolethal distending toxin subunit CdtB causes host cell cycle arrest and death.

October 13, 2000Science

A Bacterial Toxin That Controls Cell Cycle Progression as a Deoxyribonuclease I-Like Protein

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Authors

MLMarı́a Lara-TejeroYale University JGJorge E. GalánUniversity of Nebraska–Lincoln

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Implication

Laboratory study demonstrates that bacterial CdtB acts as a deoxyribonuclease to induce cell cycle arrest in cultured cells, suggesting a direct enzymatic mechanism for pathogen cytotoxicity.

Key Points

To identify the enzymatic mechanism by which the Campylobacter jejuni cytolethal distending toxin subunit CdtB causes host cell cycle arrest and death.
Transiently expressed the CdtB subunit in cultured cells to evaluate effects on chromatin integrity.
Microinjected low doses of CdtB into cells to assess cytoplasmic distention and cell cycle progression.
Generated site-directed CdtB mutants targeting conserved catalytic and magnesium-binding residues found in type I deoxyribonucleases and tested mutant holotoxin activity.
Transient expression and microinjection of wild-type CdtB directly triggered cytoplasmic distention, chromatin disruption, and cell cycle arrest.
Mutations in residues critical for type I deoxyribonuclease catalysis or magnesium binding completely prevented CdtB-mediated chromatin disruption.
CDT holotoxins incorporating the mutated CdtB subunits failed to induce morphological changes or cell cycle arrest in host cells.

Cite This Study

Lara‐Tejero et al. (2000) studied this question.

synapsesocial.com/papers/69df2a76d9e0feb21c591dc5 https://doi.org/10.1126/science.290.5490.354

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Implication

Laboratory study demonstrates that bacterial CdtB acts as a deoxyribonuclease to induce cell cycle arrest in cultured cells, suggesting a direct enzymatic mechanism for pathogen cytotoxicity.

A Bacterial Toxin That Controls Cell Cycle Progression as a Deoxyribonuclease I-Like Protein

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Member takes

Implication

Key Points

Cite This Study

A Bacterial Toxin That Controls Cell Cycle Progression as a Deoxyribonuclease I-Like Protein

Discussion

Member takes

Implication

Key Points

Cite This Study

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