Improvement of Mouse Spermatozoa Freezing at ‐80°C With Ascorbic Acid 2‐Glucoside at Thawing Phase

Key Points

• This research aims to evaluate the effect of ascorbic acid 2-glucoside on mouse sperm freezing techniques.
• Spermatozoa were frozen at -80°C and thawed using ascorbic acid 2-glucoside during the thawing phase.
• Oxidative stress levels were measured to assess the impact of antioxidants.
• Quality of spermatozoa was compared between standard freezing and antioxidant-assisted methods.
• Ascorbic acid 2-glucoside significantly reduced oxidative stress during thawing.
• Spermatozoa frozen with antioxidants showed improved quality compared to those frozen without.
• The findings suggested that antioxidants may enhance the viability of thawed sperm.