What question did this study set out to answer?

To determine how physical and chemical interactions between the lipopolysaccharide O-antigen layer and outer membrane proteins influence protective antibody binding in Salmonella.

February 12, 2020SHILAP Revista de lepidopterologíaOpen Access

Outer membrane protein size and LPS O-antigen define protective antibody targeting to the Salmonella surface

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To determine how physical and chemical interactions between the lipopolysaccharide O-antigen layer and outer membrane proteins influence protective antibody binding in Salmonella.
Evaluated the protective efficacy of immunization with the trimeric porin OmpD from Salmonella Typhimurium against homologous and heterologous Salmonella Enteritidis challenges.
Performed atomistic molecular dynamics simulations to quantify the spatial footprints formed by OmpD trimers within the lipopolysaccharide O-antigen matrix and evaluate IgG Fab accessibility.
Salmonella Typhimurium OmpD trimers generated physical voids within the O-antigen layer sufficient to permit access and binding by a single IgG Fab fragment.
Immunization with Salmonella Typhimurium OmpD provided minimal cross-protection against Salmonella Enteritidis despite a single amino-acid divergence, demonstrating that non-native O-antigen pairing obstructs protective IgG binding.

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Implication

Experimental and computational study reveals that O-antigen and porin interactions govern antibody access in Salmonella, indicating native antigen pairings dictate protective immunity.

Cite This Study

Domínguez-Medina et al. (2020) studied this question.

synapsesocial.com/papers/69df7262de200760a8615239 https://doi.org/10.1038/s41467-020-14655-9

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Implication

Experimental and computational study reveals that O-antigen and porin interactions govern antibody access in Salmonella, indicating native antigen pairings dictate protective immunity.

Outer membrane protein size and LPS O-antigen define protective antibody targeting to the Salmonella surface

Key Points

Discussion

Implication

Cite This Study

Outer membrane protein size and LPS O-antigen define protective antibody targeting to the Salmonella surface

Key Points

Discussion

Implication

Cite This Study

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