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This study aims to evaluate a biotin-free approach for developing nucleic acid lateral flow tests using UV cross-linked probes.

UV Treatment Nucleic Acid Probe Without Biotin-Labeling Is Sensitive and Sufficient For The Fabrication of Nucleic Acid Lateral Flow (NALF) Strip Test

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This study aims to evaluate a biotin-free approach for developing nucleic acid lateral flow tests using UV cross-linked probes.
Non-labelled oligonucleotides were UV cross-linked to fix test and control lines on nitrocellulose membranes.
Fabrication time and costs were measured and compared to traditional biotin-labelled protocols.
Specificity tests were conducted to evaluate false positive rates.
The UV-treated NALF strips showed a threefold reduction in fabrication time and cost compared to conventional methods.
No false positive signals were detected in negative control groups, indicating high specificity.
No significant difference in performance was observed between biotin-free UV-treated strips and biotin-labelled platforms.

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Randomized trial demonstrates efficient nucleic acid lateral flow test generation, suggesting a cost-effective alternative.

Pongsuchart et al. (2013) studied this question.

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