What question did this study set out to answer?

To determine whether mammalian miR-196 regulates HOX gene expression through direct mRNA cleavage rather than translational repression.

April 22, 2004Science

MicroRNA-Directed Cleavage of HOXB8 mRNA

View Full Paper

Ask AI

Bookmark

To determine whether mammalian miR-196 regulates HOX gene expression through direct mRNA cleavage rather than translational repression.
Evaluated sequence complementarity between miR-196 and mammalian HOX cluster transcripts, specifically HOXB8, HOXC8, and HOXD8.
Assayed developing mouse embryos for diagnostic RNA cleavage fragments corresponding to miR-196 target sites.
Performed cell culture experiments to measure downregulation and cleavage-mediated repression of HOXB8, HOXC8, HOXD8, and HOXA7.
Diagnostic RNA fragments confirmed endogenous miR-196-directed cleavage of HOXB8 transcripts in mouse embryos.
Cell culture assays demonstrated downregulation of HOXB8, HOXC8, HOXD8, and HOXA7, supporting an mRNA cleavage mechanism for miR-196-mediated repression in animal cells.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

Experimental study demonstrates miR-196-directed cleavage of HOXB8 mRNA in mouse embryos, indicating posttranscriptional HOX gene regulation via RNA cleavage.

Cite This Study

Yekta et al. (2004) studied this question.

synapsesocial.com/papers/69dfed71915fa0495361518d https://doi.org/10.1126/science.1097434

View Full Paper

Ask AI

Bookmark

Implication

Experimental study demonstrates miR-196-directed cleavage of HOXB8 mRNA in mouse embryos, indicating posttranscriptional HOX gene regulation via RNA cleavage.

MicroRNA-Directed Cleavage of HOXB8 mRNA

Key Points

Discussion

Implication

Cite This Study

MicroRNA-Directed Cleavage of HOXB8 mRNA

Key Points

Discussion

Implication

Cite This Study

Also Consider

Also Consider