What question did this study set out to answer?

Identify actively translated open reading frames across the Mycobacterium tuberculosis transcriptome, particularly noncanonical and short sequences overlooked by standard computational prediction algorithms.

March 28, 2022SHILAP Revista de lepidopterologíaOpen Access

Pervasive translation in Mycobacterium tuberculosis

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Authors

CSCarol SmithNew York State Department of Health JCJill G. CanestrariNew York State Department of Health AWArcher J. WangNew York State Department of Health

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Implication

Molecular profiling reveals pervasive translation of noncanonical short open reading frames in Mycobacterium tuberculosis, suggesting an evolutionary reservoir for novel functional proteins.

Key Points

Identify actively translated open reading frames across the Mycobacterium tuberculosis transcriptome, particularly noncanonical and short sequences overlooked by standard computational prediction algorithms.
Applied ribosome profiling approaches to directly capture actively translated open reading frames (ORFs) across the M. tuberculosis genome.
Analyzed the length, Shine-Dalgarno sequence presence, and codon usage signatures of identified ORFs to assess evolutionary purifying selection.
Demonstrated pervasive translation throughout the M. tuberculosis transcriptome, identifying numerous previously unannotated ORFs that are predominantly short (encoding proteins ≤50 amino acids) and lack canonical features.
Determined that while most discovered ORFs do not display purifying selection and likely do not impact cell fitness, 90 novel ORFs (median length of 52 codons) exhibit clear hallmarks of purifying selection.

Cite This Study

Smith et al. (2022) studied this question.

synapsesocial.com/papers/69dff77ebdd89ea5318608f0 https://doi.org/10.7554/elife.73980

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Implication

Molecular profiling reveals pervasive translation of noncanonical short open reading frames in Mycobacterium tuberculosis, suggesting an evolutionary reservoir for novel functional proteins.

Pervasive translation in Mycobacterium tuberculosis

Discussion

Member takes

Implication

Key Points

Cite This Study

Pervasive translation in Mycobacterium tuberculosis

Discussion

Member takes

Implication

Key Points

Cite This Study

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