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April 16, 2026ACS OmegaOpen Access

Split-crRNA Transcription Based Dual-Recognition Cascade Amplification Assay for Sensitive and Accurate Postoperative Delirium Related MicroRNA Analysis

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Authors

LWLihua WangHLHuihui LiKWK H Wang

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Overview

This assay demonstrates sensitive miRNA detection in postoperative delirium, highlighting its diagnostic potential.

Key Points

  • The aim is to develop an ultrasensitive assay for detecting microRNAs linked to postoperative delirium.
  • Utilized a cascade amplification strategy integrating various molecular techniques.
  • Employed a dual-recognition mechanism for enhanced specificity.
  • Conducted tests in spiked human serum to assess practical performance.
  • Compared results with RT-qPCR for validation.
  • Achieved a detection limit of 0.52 fM for miRNA-193.
  • Showed a detection range from 1 fM to 100 pM.
  • Demonstrated effective discrimination of single-base mismatches.
  • Reported recovery rates of 97.3-107.2% in human serum tests.

Cite This Study

Wang et al. (2026) studied this question.

synapsesocial.com/papers/69e07bc12f7e8953b7cbd61ehttps://doi.org/10.1021/acsomega.6c01211
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