What question did this study set out to answer?

This research aims to establish the role of transcription factors GATA4 and GATA6 in regulating cardiomyocyte hypertrophy.

August 1, 2001Journal of Biological ChemistryOpen Access

The Transcription Factors GATA4 and GATA6 Regulate Cardiomyocyte Hypertrophy in Vitro and in Vivo

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Population

Cardiomyocytes (in vitro) and transgenic mice (in vivo)

Design

Preclinical

Authors

QLQiangrong LiangNew York Institute of Technology LWLeón J. De WindtHeart Failure & Transplant SWSandra A. WittPhilipps University of Marburg

Discussion

Member takes

Implication

GATA4/6 merit further mechanistic study in hypertrophy models; leaves open translation to human therapies.

Key Points

This research aims to establish the role of transcription factors GATA4 and GATA6 in regulating cardiomyocyte hypertrophy.
GATA4 and GATA6 overexpressed in cardiomyocytes using recombinant adenovirus.
Transgenic mice with 2.5-fold GATA4 overexpression were monitored for cardiac changes.
A dominant negative GATA4-engrailed repressor was used to assess GATA factor necessity in hypertrophy.
Overexpression of GATA4 or GATA6 led to a greater than 2-fold increase in cardiomyocyte surface area and total protein.
Transgenic mice displayed increased heart to body weight ratios and signs of cardiomyopathy.
Blocking GATA factor function prevented hypertrophy responses, confirming their critical role in this process.

Structured PICO

Population

Cardiomyocytes (in vitro) and transgenic mice (in vivo)

Intervention

Overexpression of GATA4 or GATA6 (via recombinant adenovirus in vitro, or transgenic 2.5-fold overexpression in vivo); dominant negative GATA4-engrailed repressor

Outcome

Cardiomyocyte hypertrophy (measured by sarcomeric organization, cell surface area, protein accumulation, heart to body weight ratio, and gene activation)surrogate

GATA4 and GATA6 are necessary and sufficient regulators of cardiomyocyte hypertrophy in vitro and in vivo.

Cite This Study

Liang et al. (2001) studied this question.

synapsesocial.com/papers/69e1cb8f3e490517ce86b76d https://doi.org/10.1074/jbc.m102174200

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The Transcription Factors GATA4 and GATA6 Regulate Cardiomyocyte Hypertrophy in Vitro and in Vivo

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

The Transcription Factors GATA4 and GATA6 Regulate Cardiomyocyte Hypertrophy in Vitro and in Vivo

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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