Standardized method measures CO₂ concentration from nematodes, indicating metabolic activity implications.
This study aimed to standardize a method for measuring the concentration of CO ₂ released by infective juveniles (IJ's), in order to assess their metabolic activity. The nematodes used in this experiment were Heterorhabditis amazonensis JPM4 and Steinernema carpocapsae All. A standardized gas chromatography (GC) method was used for CO 2 analysis. There was a linear increase in CO 2 concentration associated with increased numbers of IJs. Additionally, the CO 2 concentration obtained for S. carpocapsae was higher than that obtained for H. amazonensis . The standardized GC method was adequate to measure the concentration of CO 2 released by IJ's.
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Sabino et al. (2015) studied this question.
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