High-throughput screening to engineer optimal T cell therapies: current knowledge and future prospects

Adoptive T cell therapy (ACT) is a potent strategy in cancer immunotherapy, but its clinical efficacy is often limited by primary resistance. To overcome this challenge, high-throughput screening technologies have emerged as essential tools for optimizing ACT. By enabling the identification of biologically significant targets and substances from vast libraries, these technologies have accelerated the development of advanced ACT strategies. This review delves into the latest advancements in high-throughput screening, highlighting its applications in genetic screening of T cells and tumor cells, as well as non-genetic screening for small molecules and targeted delivery systems. These insights provide valuable guidance for future research and clinical applications of ACT.