Whole genome sequence datasets of Priestia megaterium species and Pseudomonas machongensis from the cowpea rhizosphere

Three bacterial strains, Priestia megaterium F2, Priestia megaterium Q2, and Pseudomonas machongensis E2, were isolated from the rhizosphere of cowpea ( Vigna unguiculata L.). Genomic DNA was extracted from pure cultures and sequenced using the Illumina NovaSeq 6000 platform. Raw reads were quality-checked and assembled into draft genomes using standard bioinformatics pipelines. The assembled genome sizes were 5,696,169 bp for strain E2 (GC content 63.1%), 5,737,221 bp for strain F2 (GC content 37.6%), and 5,068,091 bp for strain Q2 (GC content 38.2%). Genome annotation was performed using automated annotation tools, identifying 5,144, 6,011, and 5,317 genes in E2, F2, and Q2, respectively. The dataset includes assembled genome sequences and corresponding annotations, with predicted genes associated with environmental adaptation, nutrient acquisition, and microbial interactions [ 1 ]. Biosynthetic gene cluster analysis identified terpene, non-ribosomal peptide synthetase (NRPS), NRPS-like, and phosphonate-associated clusters across the genomes. These data provide genomic information relevant to rhizosphere-associated bacteria isolated from cowpea under drought-prone conditions.