Phylogenetic analysis reveals Hoya lockii’s evolutionary position among Apocynaceae, suggesting significant genomic changes.
Here we report the first chloroplast genome of Hoya lockii V.T. Pham & Aver., sequenced as a circular DNA molecule (177,063 bp) comprising a large single-copy region (91,635 bp), a small single-copy region (2,242 bp), and two inverted repeats (41,593 bp), with a GC content of 36.9%. A total of 144 genes were annotated, including 98 protein-coding, 38 tRNA, and eight rRNA genes. Phylogenetic reconstruction based on 79 protein-coding genes from 50 Apocynaceae species placed H. lockii as sister to Hoya exilis with strong bootstrap support (93%). These results indicate pronounced SSC contraction and IR expansion, consistent with Hoya evolution.
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Nguyen et al. (2026) studied this question.
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