Single‐cell RNA sequencing and high‐dimensional flow cytometry reveal distinct peripheral immune landscapes of type 1 autoimmune pancreatitis and pancreatic ductal adenocarcinoma

Chenxiao Liu; Tianyi Che; Airu Liu; Yì Wáng; Qidi Yang; Yiwen Tu; Zonghao Liu; Xiaonan Shen; Xiangyi He; Tingting Gong; Ling Zhang; Zhengji Song; Junjie Fan; Yue Zeng; Wenbin Zou; Youqiong Ye; Yao Zhang; Minmin Zhang; Duowu Zou; Chunhua Zhou

doi:10.1002/ctm2.70680

Key Points

The research aims to identify distinct immune cell subsets in patients with autoimmune pancreatitis and their diagnostic significance.
Utilized single-cell RNA sequencing (scRNA-seq) combined with B cell receptor sequencing (BCR-seq) and flow cytometry.
Analyzed peripheral blood mononuclear cells (PBMCs) from patients diagnosed with autoimmune pancreatitis and pancreatic ductal adenocarcinoma.
Developed a nomogram based on flow cytometry data to differentiate between patient groups.
Identified three novel immune cell subsets in the PBMCs of autoimmune pancreatitis patients.
Demonstrated the diagnostic and prognostic value of these immune subsets.
Created a flow cytometry-derived nomogram that effectively distinguishes autoimmune pancreatitis from pancreatic ductal adenocarcinoma.

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Implication

Integration of single-cell RNA sequencing and flow cytometry identifies novel immune subsets in patients, suggesting diagnostic advances.

Abstract

By integrating scRNA/BCR-seq and flow cytometry, we identified three novel immune cell subsets in PBMCs of AIP patients and confirmed their diagnostic and prognostic value. A flow cytometry-derived nomogram based on these subsets provides a novel tool for differentiating patients with AIP from those with PDAC.

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Liu et al. (2026) studied this question.

synapsesocial.com/papers/69e9b80e85696592c86eb75b https://doi.org/10.1002/ctm2.70680

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Single‐cell RNA sequencing and high‐dimensional flow cytometry reveal distinct peripheral immune landscapes of type 1 autoimmune pancreatitis and pancreatic ductal adenocarcinoma

Key Points

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Cite This Study

Single‐cell RNA sequencing and high‐dimensional flow cytometry reveal distinct peripheral immune landscapes of type 1 autoimmune pancreatitis and pancreatic ductal adenocarcinoma

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

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