Metabolites function as real‐time indicators of physiological state, yet their rapid and precise quantification remains challenging due to chemical diversity and dynamic fluctuations. Nanopore sensing addresses these challenges by providing label‐free, single‐molecule electrical detection with high sensitivity and temporal resolution. This review systematically examines sensing strategies—from carbohydrates to neurotransmitter metabolites—for key metabolite classes. We evaluate analytical performance (sensitivity, limits of detection, temporal resolution) and compatibility with complex biofluids, chart the field's progression from single‐analyte detection toward multiplexed metabolomics, and discuss sensor stability and engineering steps required to translate proof‐of‐concept platforms into reproducible assays for clinical diagnostics and metabolic monitoring.
Zheng et al. (Wed,) studied this question.