What question did this study set out to answer?

Identify the ARC3 gene and characterize the subcellular localization and structure of the ARC3 protein during chloroplast division in Arabidopsis thaliana.

August 15, 2004Plant and Cell PhysiologyOpen Access

ARC3, a Chloroplast Division Factor, is a Chimera of Prokaryotic FtsZ and Part of Eukaryotic Phosphatidylinositol-4-phosphate 5-kinase

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Identify the ARC3 gene and characterize the subcellular localization and structure of the ARC3 protein during chloroplast division in Arabidopsis thaliana.
Sequenced the ARC3 gene from Arabidopsis thaliana mutants exhibiting defective chloroplast replication.
Assessed ARC3 protein localization, solubility, and topology using immunofluorescence microscopy, Western blotting, in vitro chloroplast import, and protease protection assays.
Sequencing identified ARC3 as a chimeric fusion between prokaryotic FtsZ and a eukaryotic phosphatidylinositol-4-phosphate 5-kinase (PIP5K) domain containing a MORN repeat motif without catalytic activity.
ARC3 was shown to be a soluble protein that assembles into a ring-like division structure on the outer surface of the chloroplast during early division stages.

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Implication

Molecular analysis reveals ARC3 as a chimeric division factor assembling on chloroplast surfaces in Arabidopsis thaliana, suggesting endosymbiotic gene evolution.

Cite This Study

Shimada et al. (2004) studied this question.

synapsesocial.com/papers/69ebfad9917f965914514f0a https://doi.org/10.1093/pcp/pch130

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Implication

Molecular analysis reveals ARC3 as a chimeric division factor assembling on chloroplast surfaces in Arabidopsis thaliana, suggesting endosymbiotic gene evolution.

ARC3, a Chloroplast Division Factor, is a Chimera of Prokaryotic FtsZ and Part of Eukaryotic Phosphatidylinositol-4-phosphate 5-kinase

Key Points

Discussion

Implication

Cite This Study

ARC3, a Chloroplast Division Factor, is a Chimera of Prokaryotic FtsZ and Part of Eukaryotic Phosphatidylinositol-4-phosphate 5-kinase

Key Points

Discussion

Implication

Cite This Study

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