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April 26, 2026AgriFood Journal of Agricultural Products for Food0 citationsOpen Access

Glucosinolate and Isothiocyanate Profiles in Different Varieties of Raphanus sativus Seed via Multivariate Data Analysis

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YLYonghua LiDLDongmei LinPWPeng Wu

Key Points

  • This research aims to quantify glucosinolates and isothiocyanates in various Raphanus sativus seed varieties to understand their profiles better.
  • Analyzed 105 varieties of Raphanus sativus seeds using high performance liquid chromatography–mass spectrometry.
  • Performed multivariate statistical analysis on the collected data.
  • Glucosinolates levels ranged from 3.59 ± 0.43 to 206.89 ± 25.11 μmol/g DW across samples.
  • Isothiocyanates levels varied from 5.6 ± 0.05 to 193.25 ± 12.86 μmol/g DW, with white RS showing notably higher levels.
  • Xuebawang was identified as a variant with particularly high functional component levels.

Abstract

ABSTRACT Raphanus sativus seed (RS) are recognized for their ability to eliminate phlegm and improve digestion in Traditional Chinese Medicine treatments. Modern medical research has revealed that RS is rich in bioactive components. However, the levels of these compounds in different RS samples remain unclear. Thus, this study, using high performance liquid chromatography–mass spectrometry, conducted a quantitative analysis of four functional constituents in 105 varieties of RS. The data were then analyzed using multivariate statistical analysis. Results indicated that the levels of glucosinolates (3.59 ± 0.43 to 206.89 ± 25.11 μmol/g DW) and isothiocyanates (5.6 ± 0.05 to 193.25 ± 12.86 μmol/g DW) varied significantly among the different RS samples, with white RS showing higher levels of glucoraphanin, sulforaphane, and sulforaphene than the other three types. Furthermore, “ Xuebawang ” was identified as a potential variant according to principal component analysis, with high levels of functional components and a relatively heavy weight (1.53 g). This study could provide preliminary data for the selection of raw materials for radish products.

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Cite This Study

Li et al. (2026) studied this question.

synapsesocial.com/papers/69edac074a46254e215b3d09https://doi.org/10.1002/agf2.70020
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