Nanoscale Protonation Limits and Charge Density in Polymer Films Govern the Activity of Immobilized LacZ under Acid Stress

Huida Duan; Junxing Chen; Felicia Fianu; Wei Sun; Yifan Cheng

doi:10.1039/d5nr05409e

January 1, 2026NanoscaleOpen Access

Nanoscale Protonation Limits and Charge Density in Polymer Films Govern the Activity of Immobilized LacZ under Acid Stress

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Key Points

This research investigates how design parameters of polymer coatings affect the activity of immobilized LacZ under acidic conditions.
Designed polycationic polymer coatings with varying protonation levels and charge densities.
Assessed enzyme activity of immobilized LacZ under controlled acid stress conditions.
Analyzed the relationship between polymer characteristics and enzyme stability.
Significant decrease in LacZ activity noted at high acidity levels, correlating with protonation limits.
Optimal charge density enhanced enzyme function, with greater stability observed in less acidic environments.
Findings suggest specific polymer design can mitigate acid stress effects on enzyme activity.

Implication

Evaluates how polymer design influences enzyme function under acidic conditions, suggesting new ways to enhance stability.

Abstract

Under acidic conditions, polycationic polymer coatings can serve as protective immobilization matrices that buffer local acidity and help preserve enzyme function. However, it remains unclear how polymer support design parameters,...

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Cite This Study

Duan et al. (2026) studied this question.

synapsesocial.com/papers/69edacbd4a46254e215b47db https://doi.org/10.1039/d5nr05409e

View Full Paper

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Nanoscale Protonation Limits and Charge Density in Polymer Films Govern the Activity of Immobilized LacZ under Acid Stress

Key Points

Implication

Abstract

Discussion

Cite This Study

Nanoscale Protonation Limits and Charge Density in Polymer Films Govern the Activity of Immobilized LacZ under Acid Stress

Key Points

Implication

Abstract

Discussion

Cite This Study

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