Demonstrates effective detection of pseudorabies virus in clinical samples, suggesting improved diagnostics.
We developed a nucleic acid detection method for pseudorabies virus (PRV) by integrating recombinase-aided amplification (RAA) with the CRISPR/Cas12a system. The assay achieved a limit of detection (LOD) of 5×10 0 copies μL -1 for recombinant plasmid and 7.96×10 -2 TCID 50 per reaction for the virus within 50 minutes. This method provides strong support for the clinical diagnosis and field surveillance of PRV.
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Li et al. (2026) studied this question.
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