Aspimultiols B and C, Two New Steroids From the Leaves and Rhizomes of Aspidistra multiflora

Two new polyhydroxylated steroids, including one spirostane‐type ( 1 ) and one pregnane‐type ( 2 ) skeleton, together with three known analogs, (25 R )‐spirost‐5‐en‐3 β ‐ol ( 3 ), (25 S )‐5 β ‐spirostane‐3 β ‐ol (4) , and (25 R )‐spirostane‐1 β ,3 β, 4 β ,5 β ‐tetraol ( 5 ), were isolated from Aspidistra multiflora . For the first time, a pregnane steroid 2 was declared from the Aspidistra genus and steroids 3 and 4 were announced from the A. multiflora species. Their chemical structures were elucidated by using IR, UV‐Vis, NMR, and HRMS‐ESI spectroscopic assessments. The cytotoxic effects on MCF‐7 and HeLa cells, as well as the nitric oxide (NO) inhibitory properties in RAW264.7 cells, of all purified compounds were evaluated. Compounds 2 and 5 meaningfully expressed the NO inhibition (IC 50 respective values meant of 30.75 and 84.93 μm).