Propofol upregulates ANGPTL4 gene and protein expression to inhibit biological behavior of colon cancer cells

Figures

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  Fig. 1. Propofol inhibits the growth of intraperitoneal colon cancer implantation in mice. (A) Images from the in vivo fluorescence imaging system. (B) Quantitative analysis of fluorescence photon intensity in the Saline and Propofol groups. (C) Comparative images of intestinal tumors in mice. (D) Comparison of tumor tissue weights. **P < 0.01.

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  Fig. 2. Propofol inhibits the proliferation, migration, and invasion of CT26.WT cells. CCK-8 assay for cell viability of CT26.WT cells treated with propofol for (A) 24 h and (B) 48 h. Transwell assay for (C,E) the migratory and (D,F) invasive abilities of CT26.WT cells treated with propofol for 24 h. ****P < 0.0001, ***P < 0.001, **P < 0.01.

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  Fig. 3. Propofol upregulates the ANGPTL4 gene in CT26.WT cells. (A) Heatmap for DEGs in CT26.WT cells treated with propofol and saline groups, with red dots representing upregulated genes, gray dots representing genes with no expression difference, and blue dots representing downregulated genes. (B) Heatmap clustering that visually describes the differences in gene expression levels, with red indicating higher expression levels and blue indicating lower expression levels. (C) GO analysis results of DEGs. (D) Expression levels of the ANGPTL4 gene in the propofol and saline groups.

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  Fig. 4. Construction of ANGPTL4 gene knockdown CT26 stable cell lines. (A) Expression levels and (B) quantitative analysis of the ANGPTL4 protein in three groups of cells; (C) mRNA expression levels of ANGPTL4 in CT26 + shRNA.NC cells and CT26 + shRNA.ANGPTL4 cells. **P < 0.01, *P < 0.05. (D) Detection of mycoplasma contamination in ANGPTL4 gene knockdown CT26 stable cell lines using a mycoplasma detection kit (PCR method).

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  Fig. 5. Effects of different concentrations of propofol on ANGPTL4 protein expression in three cell groups. The effect of different concentrations (5, 10, 15 µg/ml) of propofol on ANGPTL4 protein expression and quantitative analysis in (A,B) CT26.WT cells, (C,D) CT26 + shRNA.NC cells, and (E,F) CT26 + shRNA.ANGPTL4 cells. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05.

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  Fig. 6. Changes in proliferation, migration, and invasion of CT26 + shRNA.NC and CT26 + shRNA.ANGPTL4 cells treated with propofol. Changes in (A) proliferation, (B,C) migration, and (D,E) invasion of CT26 + shRNA.NC cells after 24 h of propofol treatment. Changes in (F) proliferation, (G,H) migration, and (I,J) invasion of CT26 + shRNA.ANGPTL4 cells after 24 h of propofol treatment. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05.