Key result
Knockdown of ANGPTL4 by siRNA in colorectal cancer cells inhibited cell proliferation, promoted apoptosis, suppressed migration and invasion, and increased sensitivity to cisplatin.
Why the study?
The regulatory role and mechanism of Angiopoietin-like protein 4 in colorectal cancer metastasis remained to be elucidated.
Does knockdown of ANGPTL4 suppress the development and metastasis of colorectal cancer in vitro?
Does knockdown of ANGPTL4 suppress the development and metastasis of colorectal cancer in vitro?
Knockdown of ANGPTL4 suppresses colorectal cancer cell proliferation, migration, and invasion while increasing sensitivity to Cisplatin, highlighting it as a potential therapeutic target.
ANGPTL4 knockdown merits further study in colorectal cancer; leaves open translation from in vitro results to clinical benefit.
Colorectal cancer, is the growth of cancer cells in the part of the colon. Angiopeptin is one of the growth factors in the human body that is particularly effective in the regulatory process. In this research, the regulatory role and its mechanism of Angiopoietin-like protein 4 (ANGPTL4) in colorectal cancer (CRC) metastasis, has been studied. Protein expression of ANGPLT4 was analyzed by immunohistochemistry in tumor samples and adjacent normal specimens of 40 patients with CRC cancer of various phases. A gene knockout test was conducted, two effective siRNA of ANGPTL4, named siRNA1 and siRNA2, were constructed and transfected into two CRC cell lines SW480 and HT-29 to block the expression of ANGPTL4. QRT-PCR and western blotting were used to validate the knockdown efficiency of the mRNA and proteins. Based on the results, the protein expression of ANGPTL4 was increased in human CRC tissues with the development of CRC. Knockdown of ANGPTL4 by siRNA in SW480 and HT-29 cells in vitro inhibited cell proliferation, promoted cell apoptosis, and suppressed the ability of cell migration and invasion. Besides, the sensitivity of CRC cells to Cisplatin was increased in the low ANGPTL4 expression group. ANGPTL4 might be a new potential therapeutic target for patients with CRC.
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Wen et al. (2020) studied Colorectal cancer (n=40). ANGPTL4 knockdown via siRNA vs. Negative control siRNA or blank control was evaluated on Cell proliferation, apoptosis, migration, and invasion. Knockdown of ANGPTL4 by siRNA in colorectal cancer cells inhibited cell proliferation, promoted apoptosis, suppressed migration and invasion, and increased sensitivity to cisplatin.
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