Key result
Higher ANGPTL4 expression correlates with advanced colorectal cancer stage and promotes tumor cell proliferation and migration.
Why the study?
The roles of angiopoietin-like proteins (ANGPTLs) in colorectal cancer remain controversial and warrant further research.
What are the expression profiles and prognostic values of ANGPTLs in colorectal cancer, and does ANGPTL4 overexpression promote proliferation and migration of colorectal cancer cells?
What are the expression profiles and prognostic values of ANGPTLs in colorectal cancer, and does ANGPTL4 overexpression promote proliferation and migration of colorectal cancer cells?
High expression of specific ANGPTLs is associated with advanced tumor stage and poorer survival in colorectal cancer, with ANGPTL4 driving cancer cell proliferation and migration and serving as a potential druggable target.
Answers are grounded in this paper and cite their sources.
May identify ANGPTL4 as a colorectal cancer target; hypothesis-generating from cell models and requires clinical validation.
Colorectal cancer (CRC) is one of the most common malignant tumors in the world. CRC recurrence and metastasis cause poor prognosis. ANGPTLs (angiopoietin-like proteins) are a family of proteins that are widely involved in metabolic disease and tumorigenesis. The roles of ANGPTLs in CRC are still controversial and deserve further research. In this study, several databases were employed to explore the expression profiles, prognostic values, genetic alterations, potential biological function, and immune infiltration correlation of ANGPTLs in CRC. The expression of ANGPTL4 was significantly positively correlated with the stage of CRC. Therefore, cell and molecular experiments were further performed to explore the roles of ANGPTL4. Our results showed that the transcriptions of ANGPTLs in colon cancer and rectal cancer tissues were lower than those in normal tissues, but the protein expression varied among different ANGPTLs. In addition, the high expression of ANGPTLs led to a relatively poor oncological outcome. Specifically, the expression of ANGPTL4 is significantly positively correlated with the stage of CRC. Further investigation revealed that ANGPTLs are mainly involved in signal transduction and the regulation of transcription, while KEGG pathway analyses demonstrated pathways in cancer. Additionally, we also observed that ANGPTL4 could promote the proliferation and migration of CRC cells, and four specific small molecule compounds had potential ANGPTL4-binding capabilities, suggesting the clinical application of these small molecule compounds on CRC treatment. Our findings imply the prognostic values and potential therapeutic targets of ANGPTLs in CRC.
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Zhang et al. (2022) studied Colorectal cancer (CRC) (n=4,535). ANGPTL1-7 expression and ANGPTL4 lentiviral overexpression vs. Normal colon/rectal tissues, low ANGPTL expression groups, or empty vector control (CMV-F) was evaluated on Correlation of ANGPTL4 expression with clinical stage of CRC, overall survival, disease-free survival, and in vitro cell proliferation and migration (p=<0.01). ANGPTL4 expression positively correlated with colorectal cancer clinical stage (p < 0.01) and promoted the proliferation and migration of HCT116 and HT29 colorectal cancer cells.
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