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This research focuses on enhancing the characterization of intact proteins, particularly biopharmaceuticals, through improved analytical techniques.

April 29, 2026Journal of the American Society for Mass Spectrometry1 citationsOpen Access

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From Congestion to Clarity: On the Complementarity of Resolving Power and Spectral Simplification for Intact Protein Characterization

LLLinda Lieu JHJingjing Huang JKJake Kline

Key Points

This research focuses on enhancing the characterization of intact proteins, particularly biopharmaceuticals, through improved analytical techniques.
Analyzed ∼25 kDa subunits of the NIST monoclonal antibody using advanced liquid chromatography techniques.
Implemented spectral simplification methods to enhance resolution and clarity of data.
Achieved a resolution increase of 97.7% with the application of spectral simplification techniques.
Successfully characterized biopharmaceutical components through improved analysis, leading to clearer data interpretation.

Abstract

200) and further to 97.7% when PTCR was applied. This approach was applied to the characterization of biopharmaceuticals by analyzing the three digested and disulfide-reduced ∼25 kDa subunits of the NIST monoclonal antibody (mAb) on a liquid chromatography time scale.

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From Congestion to Clarity: On the Complementarity of Resolving Power and Spectral Simplification for Intact Protein Characterization

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Abstract

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