Demonstrates a novel method for detecting tyrosinase activity using antifouling quantum dots, suggesting effective application in complex biological samples.
= 0.980 Å), effectively inhibiting the adsorption of various hydrophilic biomacromolecules. Leveraging the excellent antifouling performance of PEG-SQDs, we developed a strategy for detecting tyrosinase (TYR) activity. This strategy is based on TYR-catalyzed oxidation of l-tyrosine to melanin-like products, which efficiently quench the fluorescence of PEG-SQDs. Ultimately, a highly sensitive, selective, simple, and environmentally friendly method for TYR detection has been constructed. This method exhibits a linear response range for TYR from 0.5 to 20 U/mL, with a detection limit of 0.08 U/mL (S/N = 3). Furthermore, it enables accurate quantification of TYR activity in human serum samples, underscoring the excellent antifouling capability of PEG-SQDs in complex biological matrices. Overall, this work not only provides a novel tool for the sensitive detection of TYR but also offers new insights into the antifouling mechanism of SQDs.
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Huang et al. (2026) studied this question.
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