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April 29, 2026Communications BiologyOpen Access

Cytometric proteome profiling of GFP-tagged yeast for characterizing novel antifungals

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Authors

EGEslam GhazyVBVictoria BidiukFRFedor Ryabov

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Overview

Randomized trial demonstrates cellular responses to antifungal compounds in yeast, revealing novel mechanisms.

Key Points

  • This research aims to characterize the cellular responses of yeast to novel antifungal compounds through proteome analysis.
  • Assayed abundance changes in 64 GFP-tagged sentinel proteins in Saccharomyces cerevisiae using flow cytometry.
  • Monitored responses to classical and novel antifungals, focusing on mitochondrial proteins and cell cycle perturbations.
  • Analyzed effects of alkylated nucleosides on aromatic amino acid biosynthesis and oxidative stress.
  • Flow cytometry revealed expected responses for known antifungals and novel mechanisms for other compounds.
  • Alkylated nucleosides elevated proteins linked to aromatic amino acid biosynthesis; deficiencies in this pathway enhanced antifungal efficacy.
  • Antioxidants decreased the effectiveness of antifungals, dependent on aromatic amino acid biosynthesis.

Cite This Study

Ghazy et al. (2026) studied this question.

synapsesocial.com/papers/69f1a08eedf4b4682480715chttps://doi.org/10.1038/s42003-026-10083-5
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