Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
September 21, 2005Blood

A novel Syk-dependent mechanism of platelet activation by the C-type lectin receptor CLEC-2

View Full Paper
Ask AI
Bookmark
Share

Authors

KSKatsue Suzuki‐InoueUniversity of Yamanashi

Discussion

Loading...

Member takes

Implication

Experimental study reveals that CLEC-2 drives rhodocytin-induced platelet activation via Syk and PLCgamma2 in murine models, highlighting a novel C-type lectin signaling pathway.

Key Points

  • Identify the platelet receptor and downstream signaling cascade responsible for rhodocytin-mediated platelet activation independent of integrin alpha2beta1, GPIbalpha, and GPVI.
  • Purified platelet membrane proteins using rhodocytin affinity chromatography and expressed candidate receptor CLEC-2 in a cell line.
  • Assessed tyrosine phosphorylation of CLEC-2 and downstream effectors in response to rhodocytin, anti-CLEC-2 antibodies, and other agonists with or without Src family kinase inhibitor PP2.
  • Examined rhodocytin-stimulated platelet activation in genetically modified mice lacking Syk, PLCgamma2, LAT, SLP-76, or Vav1/Vav3.
  • Identified CLEC-2 as a platelet C-type lectin receptor containing a cytosolic YXXL motif that undergoes tyrosine phosphorylation specifically upon stimulation by rhodocytin or CLEC-2 antibodies.
  • Demonstrated that CLEC-2 tyrosine phosphorylation requires Src family kinases, while rhodocytin-induced platelet activation is completely abolished in Syk-deficient and PLCgamma2-deficient murine platelets.
  • Observed partial reductions in rhodocytin-induced platelet activation in mice lacking adapter proteins LAT, SLP-76, or Vav1/Vav3.

Cite This Study

Katsue Suzuki‐Inoue (2005) studied this question.

synapsesocial.com/papers/69f2f0c2e4cdef8ca70fcafdhttps://doi.org/10.1182/blood-2005-05-1994
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Aggretin, a novel platelet-aggregation inducer from snake (Calloselasma rhodostoma) venom, activates phospholipase C by acting as a glycoprotein Ia/IIa agonist1995 · 95 citations
  2. 2Linking genome and proteome by mass spectrometry: Large-scale identification of yeast proteins from two dimensional gels1996 · 1,438 citations
  3. 3Endothelial cells, megakaryoblasts, platelets and alveolar epithelial cells express abundant levels of the mouse AA4 antigen, a C-type lectin-like receptor involved in homing activities and innate immune host defense2001 · 33 citations
  4. 4Tyrosine Phosphorylation of the Fc Receptor γ-Chain in Collagen-stimulated Platelets1996 · 224 citations
  5. 5Related signals for endocytosis and basolateral sorting of the asialoglycoprotein receptor.1993 · 83 citations