What question did this study set out to answer?

To evaluate whether the chronic phase of dextran sulphate sodium (DSS)-induced colitis is driven by a Th1 or Th2 response and how this immune profile influences mucosal regeneration.

December 1, 1998Clinical & Experimental ImmunologyOpen Access

Chronic experimental colitis induced by dextran sulphate sodium (DSS) is characterized by Th1 and Th2 cytokines

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Authors

LDLevinus A. DielemanUniversity of Alberta MPMary PalmenAurinia (Canada)HAHalil AkolMeander Medisch Centrum

Discussion

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Implication

Experimental study reveals mixed Th1 and Th2 cytokine activation during incomplete colonic healing in mice, suggesting dual immune pathways impair mucosal repair.

Key Points

To evaluate whether the chronic phase of dextran sulphate sodium (DSS)-induced colitis is driven by a Th1 or Th2 response and how this immune profile influences mucosal regeneration.
Swiss Webster mice received 5% DSS in drinking water for 7 days followed by 2 to 5 weeks of normal water, with untreated mice serving as controls.
Colons were evaluated at 3 and 6 weeks post-induction via histological scoring for inflammation, tissue damage, and regeneration, alongside immunohistochemical staining for macrophages, CD4+ T cells, IFN-gamma, IL-4, and IL-5.
Two weeks after DSS cessation, colonic epithelium exhibited incomplete healing with persistently elevated total colitis scores, predominantly localized to the distal colon.
Incompletely healed regions demonstrated macrophage and CD4+ T-cell infiltration in the lamina propria, displaying focal upregulation of both IFN-gamma and IL-4 compared to control animals.
Focal epithelial regeneration defects and elevated Th1 and Th2 cytokine staining persisted in a subset of mice at 5 weeks post-DSS cessation.

Cite This Study

Dieleman et al. (1998) studied this question.

synapsesocial.com/papers/69f4097eb2dc173b842b7e63 https://doi.org/10.1046/j.1365-2249.1998.00728.x

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Implication

Experimental study reveals mixed Th1 and Th2 cytokine activation during incomplete colonic healing in mice, suggesting dual immune pathways impair mucosal repair.

Chronic experimental colitis induced by dextran sulphate sodium (DSS) is characterized by Th1 and Th2 cytokines

Discussion

Member takes

Implication

Key Points

Cite This Study

Chronic experimental colitis induced by dextran sulphate sodium (DSS) is characterized by Th1 and Th2 cytokines

Discussion

Member takes

Implication

Key Points

Cite This Study

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