Do antioxidants from medicinal plants protect cardiac myoblasts H9c2 against doxorubicin-induced cytotoxicity?
Phyllanthus emblica ethanolic extract significantly protects H9c2 cardiac myoblasts against doxorubicin-induced cytotoxicity in vitro.
Abstract: Doxorubicin is an important and effective anticancer drug widely used for the treatment of various types of cancer but its clinical use is limited by dose‐dependent cardiotoxicity. Elevated tissue levels of cellular superoxide anion/oxidative stress are a mechanism by which doxorubicin‐induced cardiotoxicity. Selected medicinal plant extracts were tested for their antioxidant capacity and cardioprotective effect against doxorubicin‐induced cardiotoxicity. The cardiac myoblasts H9c2 were incubated with the antioxidants ascorbic acid, trolox, N‐acetylcysteine or selected medicinal plant extracts including; 1) ethanolic extracts from Curcuma longa L‐EtOH Phyllanthus emblica L‐EtOH, and Piper rostratum Roxb‐EtOH; and 2) water extracts from Curcuma longa L‐H 2 O and Morus alba L‐H 2 O. The cardioprotective effects of these extracts were evaluated by crystal violet cytotoxicity assay. IC50s of doxorubicin were compared in the presence or absence of ascorbic acids, trolox, N‐acetylcysteine or plant extracts. Morus alba L‐H 2 O showed the highest antioxidant properties evaluated by ferric reducing/antioxidant power assay. Ascorbic acid and N‐acetylcysteine had modest effects on the protection of doxorubicin‐induced cytotoxicity while trolox showed insignificant protective effect. All plant extracts protected cardiac toxicity at different degrees except that Curcuma longa L‐EtOH had no protective effect. Phyllanthus emblica ‐EtOH (100 μg/ml) showed the highest cardioprotective effect (∼12‐fold doxorubicin IC50 increase). The data demonstrate that antioxidants from natural sources may be useful in the protection of cardiotoxicity in patients who receive doxorubicin.
Wattanapitayakul et al. (Sat,) studied this question.