Identification of An Optimized Collagen Extraction Protocol forRegenerative Medicine Application: A Comparative Studyof Eleven Methods.

Objective: Collagen plays a critical role in tissue engineering and regenerative medicine due to its biocompatibility and structural features. Identifying an optimal extraction method that preserves both functional and structural integrity remains challenging. This study aimed to compare eleven collagen extraction protocols to determine the most effective method for producing high-quality collagen. Materials and Methods: via subcutaneous transplantation in mice, followed by histological analysis at 30 days. Results: studies demonstrated normal tissue regeneration, including collagen deposition, angiogenesis, and epidermal restoration, without inflammatory response. Other samples exhibited incomplete structural features, reduced yield, or increased cytotoxicity. Conclusion: Protocol 4 provides a robust approach for extracting collagen with high structural integrity, functional stability, and excellent biocompatibility. This optimized collagen may be suitable for biomedical applications and could support further translational research for collagen-based therapeutic products.