A guide to selecting high-performing antibodies for ASM (UniProt ID: P17405) for use in western blot and immunoprecipitation

<ns3:p> The <ns3:italic>SMPD1</ns3:italic> gene encodes the acid sphingomyelinase (ASM) protein, a lysosomal enzyme essential for hydrolysing sphingomyelin into ceramide and phosphorylcholine, a key step in lipid metabolism and membrane homeostasis. Here we have characterized nine ASM commercial antibodies for western blot and immunoprecipitation using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While the use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs. </ns3:p>