Multiplex fluorescent PCR-based detection of Helicobacter pylori nucleic acid and antibiotic resistance genes in clinical stool samples: a performance evaluation from Gansu Province, China.
Randomized trial evaluates H. pylori detection and resistance in clinical stool samples, suggesting a reliable diagnostic tool for limited-resource settings.
Key Points
Evaluate the clinical performance of a multiplex fluorescent PCR kit for detecting H. pylori and antibiotic resistance in stool samples.
Enrolled 535 patients with suspected H. pylori infection.
Tested stool samples using multiplex fluorescent PCR for H. pylori DNA and resistance mutations.
Compared results against gastric biopsies cultured for H. pylori and antimicrobial susceptibility testing.
Culture confirmed H. pylori in 383/535 patients (71.6%).