ABSTRACT This study aimed to develop an eco‐friendly and efficient method for synthesizing selenium nanoparticles using purified laccase from Pleurotus floridanus . SeNPs were mycosynthesized at varying sodium selenite concentrations (5–20 mM). Dynamic light scattering analysis revealed particle sizes ranging from 30 to 80 nm, with a mean size of 54 nm and a polydispersity index of 0.843. SEM and HR‐TEM images showed predominantly spherical nanoparticles with an average size of 25 nm. Antibacterial assays demonstrated dose‐dependent inhibition against Pseudomonas aeruginosa , Escherichia coli , and Klebsiella pneumoniae , with the highest activity observed against P. aeruginosa at 20 mg/mL concentration. The antiproliferative effect on MCF‐7 breast cancer cells was also dose‐dependent, with an IC₅₀ of 222 μg/mL and up to 81.2% reduction in viability at 500 μg/mL. Western blot analysis showed marked upregulation of proapoptotic proteins Bax, Caspase 3, Caspase 9, and p53. These results highlight the potent antimicrobial and anticancer properties of SeNPs.
Ramalingam et al. (Tue,) studied this question.