Phytochemical profiling and in vitro biological activities of Opuntia ficus-indica oil extracted by different methods.

Key Points

• This research aims to explore the phytochemical composition and biological activities of Opuntia ficus-indica oil extracted by various methods.
• Tested antimicrobial activity against Staphylococcus aureus, Staphylococcus epidermidis, and Candida albicans using a disc diffusion assay.
• Compared oil extracted through different methods, particularly focusing on maceration-derived oil.
• Analyzed phytochemical profile and measured inhibition zones for each method.
• Maceration-derived oil showed the largest inhibition zones: 9.7 ± 0.3 mm against Staphylococcus aureus, 8.6 ± 0.2 mm against Staphylococcus epidermidis, and 12 ± 0.6 mm against Candida albicans.
• Seed oil demonstrated substantial antioxidant activity, indicating potential health benefits.
• Phytochemical composition analysis revealed a rich profile, supporting pharmaceutical and cosmetic applications.