Identification of Gibberellins A1, A3, and Iso-A3 in Cultures of Azospirillum lipoferum

Gibberellins A(1), A(3), and iso-A(3) were identified from aseptic cultures of Azospirillum lipoferum strain op 33 by capillary gas chromatography-mass spectrometry (GC-MS) and GC-MS-selected ion monitoring. There were 20 to 40 picograms (in GA(3) equivalents, estimated from bioassay) of gibberellins A(1) and A(3) per milliliter of cell culture (containing 10(9) cells).