The Recombinant Production of Novel Bovine Lactoferricin Engineered Peptide Using Molecular Dynamics Simulation.

Key Points

• The aim is to develop a novel bovine lactoferricin peptide through recombinant production and assess its interactions with DNA and bacterial pathogens.
• Recombinant production of a lactoferricin peptide confirmed via SDS PAGE.
• Molecular dynamics simulations were utilized to engineer the peptide structure.
• Disk diffusion methods were employed to evaluate the peptide's antibacterial activity against gram-positive and gram-negative bacteria.
• The engineered lactoferricin showed improved complex formation with DNA, DNAK, and LysM.
• Peptide effectiveness was strongest at concentrations of 6 and 8 mg/ml for gram-positive and gram-negative bacteria, respectively.
• Removal of the PHE25 residue significantly enhanced interaction capabilities.