Bioaccumulation and elimination kinetics of metals and polycyclic aromatic hydrocarbons in Hermetic illucens larvae: Implications for risk mitigation of selected contaminants in insect farming.

for each of four PAHs (benzo[a]pyrene, benz[a]anthracene, benzo[b]fluoranthene, chrysene). In the second phase, insects were exposed to non-contaminated substrates for five days to evaluate elimination. Results showed metal accumulation with kinetic bioaccumulation factors of 4.26, 1.16, and 1.31 for Cd, As, and Pb, respectively. A one-day depuration reduced As and Pb below regulatory thresholds; Cd required three days. Minimal to no accumulation was observed for B[a]P and B[b]F, with higher uptake for B[a]A. PAH half-lives were under one day. These findings, from controlled experiments with limited contaminants, support incorporating a depuration phase for risk mitigation in H. illucens waste-to-protein systems.