Design of Experiments-assisted Micellar Electrokinetic Chromatography Separation of Phosphodiesterase-5 Inhibitors: Application to Sildenafil and Tadalafil.

Key Points

• The study aims to develop and validate a method for separating phosphodiesterase-5 inhibitors using micellar electrokinetic chromatography.
• Applied design of experiments to optimize separation conditions.
• Validated the method for precision, linearity, accuracy, and robustness.
• Tested the method on pharmaceutical formulations and dietary supplements.
• Achieved migration times below 6 minutes with an analytical performance score of 4.75.
• Method showed satisfactory precision and accuracy in separating sildenafil and tadalafil.
• Confirmed suitability for routine screening and quality control in various samples.