Covalent functionalization of polymeric nanoparticles prepared from nano-emulsion templates for gene silencing and antioxidant delivery at neuronal level.

Key Points

• This research aims to explore a polymeric nanoparticle system for simultaneous gene silencing and antioxidant delivery in neuronal cells.
• Cell culture experiments conducted on HEK293 and SH-SY5Y cell lines.
• Uptake studies performed to assess the internalization of PLGA nanoparticles by SH-SY5Y cells and primary murine neurons.
• The system allows independent assessment of redox modulation and gene silencing.
• PLGA nanoparticles achieved a 53.7 ± 7.9% reduction in luciferase expression for gene silencing in SH-SY5Y cells.
• Demonstrated effective internalization in both SH-SY5Y cells and primary murine neurons.
• Confirmed biocompatibility in both non-cancer and neuroblastoma cell lines.