Assessment of between-bottle homogeneity, particle size, and element content of candidate reference materials of black cricket (Gryllus assimilis) employing energy-dispersive X-ray fluorescence spectrometry.

). Coefficients of variation were below 11%, and expanded uncertainties remained under 30%. These results demonstrate adequate precision, reproducibility, and matrix suitability of the proposed material. The study supports continued evaluation of intra-bottle homogeneity, stability, and certification to enable its availability for laboratories performing elemental analysis of cricket-based foods, strengthening analytical reliability and food safety assessment in emerging insect protein chains worldwide. This contribution addresses national needs and supports sustainable nutrition research efforts.