Randomized trial demonstrates improved white adipose tissue homeostasis in mice fed a high-fat diet, indicating a beneficial effect of Cynandione A.
Key Points
The study aims to examine the effects of Cynandione A on the function of white adipose tissue in high-fat diet-fed mice.
C57BL/6 J mice were given a high-fat diet and treated with Cynandione A (5 or 15 mg/kg) intraperitoneally once daily for eight weeks.
Body weight, glucose tolerance, insulin sensitivity, and serum parameters were measured, along with histological analyses of white adipose tissue and liver.
Gene and protein expressions related to beige adipocyte characteristics and mitochondrial biogenesis in inguinal white adipose tissue were assessed.
Treatment with CA at 15 mg/kg led to a 43.8% reduction in adipocyte size in inguinal white adipose tissue (P < .001).
Significant increases in beige adipocyte-specific gene expressions (Tmem26: 2.8-fold, Cad137: 3.8-fold, both P < .001) were observed in iWAT after CA treatment.
Mitochondrial marker proteins also increased, with UCP1 showing a 2.1-fold increase (P = .007) and TOM20 a 2.2-fold increase (P < .001).