The dual-antibody immunoassay demonstrates high reproducibility and accuracy in PDCoV antigen detection, indicating strong potential for diagnostic assays.
/100 μL, with high specificity against common porcine enteric pathogens and good reproducibility. A dual-standard calibration strategy further enabled both viral titer estimation and antigen quantification. When evaluated using 122 clinical samples, the ELISA showed a concordance rate of 95.9% with RT-qPCR (Kappa = 0.85). In addition, PDCoV shedding was detectable as early as 24 h post-infection in experimentally infected piglets. Overall, this nanobody-based ELISA provides a reliable approach for PDCoV antigen detection and highlights the potential of nanobody engineering in the development of diagnostic assays.
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Hou et al. (2026) studied this question.
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