Key result
rPDCoV-N-ELISA demonstrates ~100% sensitivity for detecting porcine deltacoronavirus antibodies.
Why the study?
Diagnostic tools for serological surveys of porcine deltacoronavirus remain in the developmental stage compared with those for other porcine coronaviruses.
Effect estimate: ROC AUC 0.962 (95% CI 0.916-1.000)
p-value: p=<0.001
The rPDCoV-N-ELISA provides a sensitive and specific diagnostic tool for serological surveys of porcine deltacoronavirus.
rPDCoV-N-ELISA shows no cross-reactivity; leaves open its utility for PDCoV serosurveys pending validation.
Recently, porcine deltacoronavirus (PDCoV) has been proven to be associated with enteric disease in piglets. Diagnostic tools for serological surveys of PDCoV remain in the developmental stage when compared with those for other porcine coronaviruses. In our study, an indirect enzyme-linked immunosorbent assay (ELISA) (rPDCoV-N-ELISA) was developed to detect antibodies against PDCoV using a histidine-tagged recombinant nucleocapsid (N) protein as an antigen. The rPDCoV-N-ELISA did not cross-react with antisera against porcine epidemic diarrhea virus, swine transmissible gastroenteritis virus, porcine group A rotavirus, classical swine fever virus, porcine circovirus-2, porcine pseudorabies virus, and porcine reproductive and respiratory syndrome virus; the receiver operating characteristic (ROC) curve analysis revealed 100% sensitivity and 90.4% specificity of the rPDCoV-N-ELISA based on samples of known status (n=62). Analyses of field samples (n=319) using the rPDCoV-N-ELISA indicated that 11.59% of samples were positive for antibodies against PDCoV. These data demonstrated that the rPDCoV-N-ELISA can be used for epidemiological investigations of PDCoV and that PDCoV had a low serum prevalence in pig population in Heilongjiang province, northeast China.
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Su et al. (2015) studied Porcine deltacoronavirus (PDCoV) infection (n=381). rPDCoV-N-ELISA vs. Western blotting was evaluated on Diagnostic accuracy (Area under the ROC curve) (ROC AUC 0.962, 95% CI 0.916-1.000, p=<0.001). The rPDCoV-N-ELISA demonstrated 100% sensitivity and 90.4% specificity (ROC AUC 0.962) for detecting antibodies against porcine deltacoronavirus, revealing an 11.59% seroprevalence in field samples.
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