To identify gene targets that enhance octanoic acid production and tolerance in Escherichia coli.
Conducted genome-scale CRISPRi screening
Analyzed gene knockout effects on octanoic acid production
Examined microbial stress tolerance mechanisms
Identified key gene targets linked to octanoic acid production
Enhanced production levels observed in modified Escherichia coli strains
Demonstrated utility of CRISPRi for genetic analysis
Abstract
strains for MCFAs production and highlights the utility of genome-scale CRISPRi screening for identifying genetic determinants of microbial stress tolerance.