regeneration. The common precursor, adipic acid, was produced by an upstream strain optimized through reverse β-oxidation pathway reconstruction, PaaJ engineering, and metabolic flux balancing, achieving a titer of 6.1 g/L. In fed-batch fermentation, cocultivation of the engineered strains with delayed inoculation enabled temporally coordinated conversion of glycerol to HMD (230.9 mg/L) and ε-CL (808.0 µg/L), representing low yet the highest titers reported to date. This work opens up the possibility of a unified, modular microbial platform for the sustainable production of nylon monomers from a renewable carbon source.
Ahn et al. (Mon,) studied this question.