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May 7, 2026Resuscitation Plus0 citationsOpen Access

Evaluation of genetic-molecular causes of Out-of-Hospital Cardiac Arrest - from patients to families: the GenetiCA study

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AMArianna MorenaSBSara BendottiADAnnapaola D’Ambrosio

Key Result

The GenetiCA study will prospectively assess the genetic burden of out-of-hospital cardiac arrest in patients aged ≤50 years, with pathogenic or likely-pathogenic variants anticipated in 15-25%.

Key Points

  • This study aims to evaluate the genetic-molecular causes of out-of-hospital cardiac arrest (OHCA) in patients aged ≤50 years.
  • Multicenter, prospective observational study in Lombardy Region, Italy.
  • Enrollment of patients aged ≤50 who experience medically-caused OHCA; ClinicalTrials.gov NCT06844851.
  • Collection of blood samples for genetic analysis using next-generation sequencing targeting arrhythmogenic syndromes and cardiomyopathies.
  • Expected P/LP variants in 15–25% of young OHCA cases, with approximately 86–144 individuals identified annually.
  • First comprehensive population-based assessment of inherited cardiac diseases among young OHCA patients in the region.
  • Findings will inform targeted family screening and public health policy implications.

Study Design

Type

Observational

Multicenter

Yes

Structured PICO

P
Population
Patients aged ≤50 years who experience a medically-caused out-of-hospital cardiac arrest (OHCA) in Lombardy Region, Italy (expected ~575 cases annually).
I
Intervention
Genetic analysis using Next-Generation Sequencing with a comprehensive multigene panel targeting inherited arrhythmogenic syndromes and cardiomyopathies.
O
Outcome
Prevalence of inherited arrhythmogenic and structural cardiomyopathies among young OHCA cases in this geographic area.

The GenetiCA study protocol outlines a prospective, population-based approach to determine the prevalence of inherited arrhythmogenic and structural cardiomyopathies in young patients with out-of-hospital cardiac arrest.

Abstract

Out-of-hospital cardiac arrest (OHCA) is a public health priority. In patients aged≤50years, inherited arrhythmogenic and structural heart diseases represent a leading etiology, yet existing evidence is largely derived from small, selected cohorts, and population-based estimates of genetic contribution to OHCA in young are still lacking. a multicenter, prospective observational study enrolling all patients aged≤50 years who experience a medically-caused OHCA in Lombardy Region (Italy). (ClinicalTrials.gov NCT06844851). Blood samples will be collected in the pre-hospital by Emergency Medical System during Advanced Life Support. Genetic analysis will be performed using Next-Generation Sequencing with a comprehensive multigene panel targeting inherited arrhythmogenic syndromes and cardiomyopathies. Pre-hospital clinical data, environmental air pollution data and clinical outcomes will be collected. Family members of probands carrying pathogenic(P), likely-pathogenic(LP), or uncertain variants will be offered genetic counseling and clinical evaluation. The primary outcome will be to determine the prevalence of inherited arrhythmogenic and structural cardiomyopathies among young OHCA cases in this geographic area. Secondary outcomes will assess regional variability, associations with initial rhythm, sex, environmental pollution, and survival according to genetic findings. Expected results: Based on the regional OHCA registry (LombardiaCaRe), approximately 575 resuscitated young OHCA cases are expected annually. P/LP variants are anticipated in 15–25% of cases, corresponding to 86–144 individuals/year. GenetiCA study will provide the first population-based assessment of inherited cardiac diseases among young OHCA patients within an entire geographic region. It aims to clarify the genetic burden of OHCA, support targeted family screening with potential implications for public health policies.

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Cite This Study

Morena et al. (2026) conducted an observational in Out-of-hospital cardiac arrest (OHCA). Next-Generation Sequencing multigene panel was evaluated on Prevalence of inherited arrhythmogenic and structural cardiomyopathies among young OHCA cases. The GenetiCA study will prospectively assess the genetic burden of out-of-hospital cardiac arrest in patients aged ≤50 years, with pathogenic or likely-pathogenic variants anticipated in 15-25%.

synapsesocial.com/papers/69fc2b608b49bacb8b347849https://doi.org/10.1016/j.resplu.2026.101351
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