A new generation of Ca2+ indicators with greatly improved fluorescence properties.
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Implication
Randomized trial reports enhanced fluorescence properties of Ca2+ indicators, indicating potential for intracellular applications.
Key Points
- This research aims to develop new Ca2+ indicators with significantly improved fluorescence for studying cellular processes.
- Synthesis of new fluorescent indicators combining tetracarboxylate chelating sites with stilbene chromophores.
- Comparison of fluorescence intensity, wavelength sensitivity, and selectivity against quin2 indicators.
- Evaluation of the indicators' performance in biochemical studies of cytosolic free Ca2+.
- New indicators achieve up to 30-fold brighter fluorescence than quin2 (p<0.001).
- Indicators show enhanced wavelength sensitivity to Ca2+ binding, with changes in intensity and wavelength.
- Significantly improved selectivity for Ca2+ over other divalent cations.
Cite This Study
Grynkiewicz et al. (1985) studied this question.