A disseminated neonatal echovirus 11 infection model was successfully established in 3-day-old hFcRn transgenic mice, offering a platform for future vaccine and therapeutic research.
copies/mg). Additionally, liver and kidney function (ALT, AST and Cr), cardiac enzymes (CK-MB), and coagulation parameters (FDP) were markedly abnormal in the E11 group, in which ALT level showed 3.5 times higher than the normal group. Furthermore, in situ hybridization revealed E11 RNA in the heart, liver, lungs, and brain, with the highest levels in the heart and liver. Serum cytokine analysis showed significant elevation of MCP-1, IL-18, IL-22, and RANTES, with MCP-1 reaching levels as high as 2590 pg/mL. Histopathological examination revealed extensive pathological changes in multiple tissues. The successful establishment of the disseminated neonatal E11 infection model relies on several key factors, including the use of 3-day-old mice, an increased dosage of the infectious agent, an appropriate infection route via IP injection, and the use of viral strains obtained from the clinical case of neonatal mortality. In summary, this mouse model serves as a valuable tool for investigating the pathogenic mechanisms of E11 infection associated disseminated neonatal infection, offering a stable platform for the development and evaluation of related vaccines and therapeutic drugs.
Li et al. (Tue,) studied this question.