What question did this study set out to answer?

To determine whether TNF alpha is expressed directly by human skeletal muscle and evaluate its relationship to whole-body insulin resistance and glucose disposal.

February 15, 1996Journal of Clinical InvestigationOpen Access

The expression of TNF alpha by human muscle. Relationship to insulin resistance.

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To determine whether TNF alpha is expressed directly by human skeletal muscle and evaluate its relationship to whole-body insulin resistance and glucose disposal.
Quantified TNF alpha expression via competitive RT-PCR in skeletal muscle biopsies from N=15 subjects previously characterized by the glucose clamp technique.
Detected TNF alpha mRNA across human heart, human and rat skeletal muscle, and cultured myocytes using RT-PCR.
Cultured vastus lateralis muscle biopsy cells from N=9 subjects for 4 weeks, differentiated them into myotubes, and measured secreted TNF alpha in the medium.
Muscle TNF alpha expression was fourfold higher in insulin-resistant and diabetic subjects compared to insulin-sensitive subjects.
Maximal glucose disposal rate demonstrated a significant inverse linear relationship with muscle TNF alpha expression (r = -0.60, P < 0.02).
Cultured differentiated myotubes from diabetic patients secreted threefold more TNF alpha into the culture medium than cells from nondiabetic controls.

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Implication

Translational analysis reveals elevated muscle TNF alpha expression in insulin-resistant and diabetic adults, suggesting a local muscular mechanism driving systemic insulin resistance.

Cite This Study

Saghizadeh et al. (1996) studied this question.

synapsesocial.com/papers/69fd29cd807917f9cf086493 https://doi.org/10.1172/jci118504

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Implication

Translational analysis reveals elevated muscle TNF alpha expression in insulin-resistant and diabetic adults, suggesting a local muscular mechanism driving systemic insulin resistance.

The expression of TNF alpha by human muscle. Relationship to insulin resistance.

Key Points

Discussion

Implication

Cite This Study

The expression of TNF alpha by human muscle. Relationship to insulin resistance.

Key Points

Discussion

Implication

Cite This Study

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