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May 4, 2026STAR ProtocolsOpen Access

Protocol for isolating and culturing human monocytes from peripheral blood and measuring intracellular calcium dynamics using Fura-2

Maria F. Cano-Abad

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Key Points

This research aims to outline a reliable protocol for isolating human monocytes and analyzing their intracellular calcium levels.
Isolated human monocytes from peripheral blood through density gradient centrifugation.
Cultured monocytes in appropriate media under sterile conditions.
Measured intracellular calcium levels using Fura-2 dye and fluorescence microscopy.
Successful isolation of viable human monocytes with high purity rates.
Demonstrated the effective measurement of intracellular calcium dynamics using Fura-2.
Established a feasible protocol for future studies on calcium signaling in immune cells.

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Implication

Protocol demonstrates measuring intracellular calcium dynamics in human monocytes, indicating a method for cellular analysis.

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Cite This Study

Maria F. Cano-Abad (2026) studied this question.

synapsesocial.com/papers/69fd7d4abfa21ec5bbf05caf https://doi.org/10.1016/j.xpro.2026.104511

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Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

1Protocol for monitoring cytoplasmic Ca2+ dynamics using Fura Red-AM on a Zeiss Axio Observer microscopeOriginal Research · 2026
2A Novel, Rapid Method to Quantify Intraplatelet Calcium Dynamics by Ratiometric Flow CytometryOriginal Research · 2015 · 29 citations
3Protocol to assess calcium signaling in response to metabolic alterations by flow cytometry and live-cell imagingOriginal Research · 2026
4How to measure intracellular [Ca2+] in single cardiac cells with fura-2 or indo-1other · 1995 · 26 citations
5In situ calibration of fura-2 and BCECF fluorescence in adult rat ventricular myocytespreclinical · 1990 · 45 citations

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