A novel in situ calibration method using La3+ for fura-2 and specific buffers for BCECF allows accurate measurement of intracellular calcium and pH in adult rat ventricular myocytes without causing hypercontracture.
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Provides calibrated [Ca2+]i and pH values in rat myocytes; extends in situ methods but leaves open human translation.
Borzak et al. (1990) studied this question.
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